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Table 3 Kinetic and stoichiometric parameters of wild type and mutant strains transformed with plasmid pHN

From: Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production

Parameter

W3110p

WGp

WGMCp

WGMp

WGMEp

WHICp

ptsG)

ptsG, Δ manX, ΔmglABC)

ptsG, Δ manX)

ptsG, Δ manX, Δ nagE)

ptsHIcrr, Δ mglABC)

Maximum biomass concentration (g/L)

1.14 ± 0.15

1.42 ± 0.02

0.82 ± 0.30

1.08 ± 0.05

1.12 ± 0.06

1.13 ± 0.16

Maximum plasmid pHN concentration (mg/L)

0.83 ± 0.18

1.06 ± 0.19

1.63 ± 0.45

1.09 ± 0.34

1.19 ± 0.41

1.47 ± 0.30

Maximum acetate concentration (g/L)

0.40 ± 0.01

0

0

0

0

0

μ (h-1)

0.63 ± 0.04

0.49 ± 0.00

0.33 ± 0.06

0.33 ± 0.02

0.37 ± 0.01

0.14 ± 0.02

YP/S (mg/g)

0.43 ± 0.15

0.54 ± 0.10

1.03 ± 0.01

0.89 ± 0.00

0.75 ± 0.05

0.84 ± 0.04

YP/X (mg/g)

0.65 ± 0.04

0.93 ± 0.14

2.10 ± 0.30

1.57 ± 0.14

1.39 ± 0.09

1.08 ± 0.13

q s (g/g h)

1.20 ± 0.07

0.89 ± 0.07

0.70 ± 0.09

0.59 ± 0.03

0.57 ± 0.03

0.31 ± 0.07

q p (g/g h)

0.50 ± 0.09

0.43 ± 0.02

0.53 ± 0.09

0.53 ± 0.05

0.51 ± 0.02

0.15 ± 0.01

Culture time

(h)

5

7

9

8

9

24