Table 2 Kinetic and stoichiometric parameters of wild type and mutant strains lacking various glucose transporters
Strains | Deleted gene(s) | μ(h-1) | q s (g/g h) | q ac (g/g h) | YX/S |
|---|---|---|---|---|---|
W3110 | - | 0.65 ± 0.02 | 1.33 ± 0.04 | 0.19 ± 0.02 | 0.49 ± 0.00 |
WG | ptsG | 0.51 ± 0.01 | 1.05 ± 0.12 | 0.07 ± 0.05 | 0.53 ± 0.12 |
WGX | ptsG, malX | 0.23 ± 0.03 | 0.51 ± 0.01 | 0 | 0.41 ± 0.01 |
WGB | ptsG, bglF | 0.43 ± 0.04 | 1.06 ± 0.03 | 0.06 ± 0.02 | 0.42 ± 0.03 |
WGE | ptsG, nagE | 0.41 ± 0.02 | 0.69 ± 0.06 | 0 | 0.60 ± 0.08 |
WGM | ptsG, manX | 0.36 ± 0.03 | 0.65 ± 0.05 | 0 | 0.56 ± 0.08 |
WGMX | ptsG, manX, malX | 0.32 ± 0.01 | 0.58 ± 0.06 | 0 | 0.55 ± 0.06 |
WGMB | ptsG, manX, bglF | 0.29 ± 0.00 | 0.66 ± 0.07 | 0 | 0.40 ± 0.02 |
WGME | ptsG, manX, nagE | 0.28 ± 0.02 | 0.56 ± 0.00 | 0 | 0.48 ± 0.03 |
WGP | ptsG, galP | 0.49 ± 0.08 | 1.19 ± 0.00 | 0.06 ± 0.00 | 0.44 ± 0.01 |
WGC | ptsG, mglABC | 0.48 ± 0.01 | 1.02 ± 0.00 | 0.08 ± 0.00 | 0.47 ± 0.05 |
WGMP | ptsG, manX, galP | 0.29 ± 0.01 | 0.56 ± 0.01 | 0 | 0.52 ± 0.02 |
WGMC | ptsG, manX, mglABC | 0.31 ± 0.01 | 0.68 ± 0.00 | 0 | 0.47 ± 0.01 |
WHI | ptsHIcrr | 0.25 ± 0.01 | 0.49 ± 0.03 | 0 | 0.51 ± 0.02 |
WHIP | ptsHIcrr, galP | 0.18 ± 0.02 | 0.32 ± 0.02 | 0 | 0.55 ± 0.03 |
WHIC | ptsHIcrr, mglABC | 0.20 ± 0.01 | 0.32 ± 0.02 | 0 | 0.38 ± 0.05 |