Table 1 E. coli strains and plasmid employed in this study

Strains

JW1087-2

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔptsG763::kan, rph-1,Δ(rhaD-rhaB)568, hsdR514

[23]

JW1806-1

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔmanX741::kan, rph-1,Δ(rhaD-rhaB)568, hsdR514

[23]

JW1613-1

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔmalX769::kan, rph-1,Δ(rhaD-rhaB)568, hsdR514

[23]

JW3700-1

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, rph-1, ΔbglF753::kan,Δ(rhaD-rhaB)568, hsdR514

[23]

JW0665-1

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔnagE728::kan, λ^-, rph-1,Δ(rhaD-rhaB)568, hsdR514

[23]

JW2910-1

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔgalP789::kan, rph-1,Δ(rhaD-rhaB)568, hsdR514

[23]

W3110

F^- λ^-rph-1 IN(rrnD-rrnE) 1

[23]

WG

W3110 ∆ptsG::FRT-Km-FRT

This study JW1087-2

WGX

WG ∆malX::FRT-Km-FRT

This study JW1613-1

WGB

WG ∆bglF::FRT-Km-FRT

This study JW3700-1

WGE

WG ∆nagE::FRT-Km-FRT

This study JW0665-1

WGM

WG ∆manX::FRT-Km-FRT

This study JW1806-1

WGMX

WGM ∆malX::FRT-Km-FRT

This study JW1613-1

WGMB

WGM ∆bglF::Km; FRT-Km-FRT

This study JW3700-1

WGME

WGM ∆nagE::FRT-Km-FRT

This study JW0665-1

WGP

WG ∆galP::FRT-Km-FRT

This study JW2910-1

WGC

WG ∆mglABC::FRT-Cm-FRT

This study mglABCDtF, mglABCDtR

WGMP

WGM ∆galP::FRT-Km-FRT

This study JW2910-1

WGMC

WGM, ∆mglABC::FRT-Cm-FRT

This study mglABCDtF, mglABCDtR

WHI

W3110 ∆ptsHIcrr::FRT-Cm-FRT

This study PTSDF PTSDR

WHIP

WHI ∆galP::FRT-Km-FRT

This study JW2910-1

WHIC

WHI ∆mglABC::FRT-Cm-FRT

This study mglABCDtF, mglABCDtR

W3110p

W3110/pHN

This study

WGp

WG/pHN

This study

WGMCp

WGMAB/pHN

This study

WGMp

WGM/pHN

This study

WGMEp

WGME/pHN

This study

WHICp

WHIAB/pHN

This study

Plasmids

pHN

Derivative of pcDNA3.1 with cloned gene encoding for a 567 nucleotide region from the HN gene

[25]

pKD3

bla, FRT-Cm-FRT

[23]

pKD46

bla, γ β exo (red recombinase), temperature-conditional replicon

[23]

pCP20

bla, flp, temperature-conditional replicon

[23]

Primers

ptsGF

CGC AGG TAA CCA CCG ATA AC

This study

ptsGR

GCA ACG CGC TAT ATT GCA GA

This study

manXF

ATC TGG CAC GTT GAC GTG TT

This study

manXR

TTG CCG TTA TCA GCA GCC TT

This study

malXF

AGC CAT GCA GAT GAC CTA CT

This study

malXR

AAC GGT CAG CGA CAT AAT CC

This study

bglFF

GGA TTG TTA CCG CAC TAA GC

This study

bglFR

AGG CAC CTT CCA CCT GAT TG

This study

nagEF

CGT TGG CGG ATT AGG CAT CT

This study

nagER

TGT TGG ATG CGA CGC TCA AG

This study

glk2F

CCG CCA GCA AGA CCG AGA AT

This study

glk2R

TCT ACC GCC GCT TCT TCC AG

This study

galPF

CGA TGC TGC CGG TCT GAA GT

This study

galPR

GTG TTG CGA CGC ACG GAT TG

This study

PTSF

CGA TGT GGC GGT AAC AAT CT

This study

PTSR

CCG CTT CAT AGC AGG TAT GT

This study

mglABF

GCT TCG GCG TTC AGT AAC AC

This study

mglABR

TAT GAC CGA ATG CGG ACC AC

This study

PTSDF

CTA GAC TTT AGT TCC ACA ACA CTA AAC CTA TAA GTT GGG GAA ATA CAA TGG TGT AGG CTG GAG CTG CTT C

This study

PTSDR

ATG GGC GCC ATT TTT CAC TGC GGC AAG AAT TAC TTC TTG ATG CGG ATA ACA TGG GAA TTA GCC ATG GTC C

This study

mglABCDtF

AGC ATT TAT CTC AAG CAC TAC CCT GCA TAA GAA AAA CCG GAG ATA CCA TGG TGT AGG CTG GAG CTG CTT C

This study

mglABCDtR

TTT ATG ACC GAA TGC GGA CCA CAT TCA CAT CAT TTC TTA CGC GCG TAT TTA TGG GAA TTA GCC ATG GTC C

This study