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Table 2 Plasmids used in this study

From: Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol

Plasmid Name

Description

Used for strain

Reference

pAM2991

NSI targeting vector

-

[28]

pZE12-

Contain C. beijerinckii adh gene codon optimized for E.coli

-

[4]

alsS-alsD-CBADH

pZE12-

Contain T. brockii adh gene codon optimized for E.coli

-

[4]

alsS-alsD-TBADH

GYM

NSI targeting. LacIq; Ptrc::gldA, yqhD, mgsA; SpecR

LH21

This work

CYM

NSI targeting. LacIq; Ptrc:: C.beijerinckii adh, yqhD, mgsA; SpecR

LH22

This work

TYM

NSI targeting. LacIq; Ptrc::T.brockii adh, yqhD, mgsA; SpecR

LH23

This work

pZElac

E.coli vector whose backbone was used to build his tag protein expression plasmids used in this study.

-

[29]

pQE-9

Vector for N-terminal 6Xhis-tagged protein expression

-

Qiagen

pZElac_his

The pZElac inserted with the T5 promoter/lacO::6Xhis part from pQE-9.

-

This work

His-gldA

E.coli vector for his tag- E.coli gldA expression.

-

This work

His-CB

E.coli vector for his tag- C. beijerinckii adh expression.

-

This work

His-TB

E.coli vector for his tag- T. brockii adh expression.

-

This work