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Figure 2 | Microbial Cell Factories

Figure 2

From: Engineering a cyanobacterium as the catalyst for the photosynthetic conversion of CO2 to 1,2-propanediol

Figure 2

Construction of the 1,2-propanediol pathways. (A) Illustration of artifical operons inserted into the Synechococcus elongatus PCC 7942 Neutral Site I (NSI) to build strains LH21, LH22, and LH23. gldA, yqhD and mgsA are from E.coli. Secondary alcohol dehydrogenases (sADHs) are from C. beijerinckii and T. brockii. (B) Test of the total RNA quality and the RT-PCR system using housekeeping gene rnpB. (C) Verification of the heterologous gene expression in engineered cyanobacteria strains by RT-PCR.

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