Figure 6
From: Stable expression plasmids for Streptomyces based on a toxin-antitoxin system
Protein production after serial subcultures. Amylase (A) and Xylanase (C) production by S. lividans ΔTA-pKC796-Tox transformed with pNAnti-Amy (A) and pNAnti-Xyl (C) after three serial subcultures every two days (P1, P2, and P3) in fresh YES medium supplemented with 3% xylose. 10 μL of 4-day supernatants was loaded in each track. B and D show the percentage of amylase (B) and xylanase (D) activity of the supernatants. The histogram bars are the mean of four experiments.