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Table 3 Nucleotide sequences of primers where N is A/T/G/C and S is C/G

From: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Primer name

Sequence (from 5′ to 3′)

5MT-Bam HI

TATGGATCCATGAGAACTTCTTCTAGACTTATCG

5MT-Xho HI

TATCTCGAGCAGAATAACGTTCCAAACACC

3MT-Xho HI

TATCTCGAGTTACAAACATTGAGAGTACC

5MT-G323X

AGTCAATGCTNNSCTTTGGTTCAAGCC

3MT-G323X

TTGAACCAAAGSNNAGCATTGACTGCG

5MT-A322X

TACCGCAGTCAATNNSGGTCTTTGGTTCAAGC

3MT-A322X

AACCAAAGACCAGCSNNGACTGCGGTACCTCC

5MT-L324X

AGTCAATGCTGGTNNSTGGTTCAAGCCATATTCTTTGG

3MT-L324X

ACCAGCATTGACTGCGGTACCSNNACCAGCAT

5MT-N700X

TCCTTCTAACAGACGTTCTNNSCACTGGATGGGTACTAAC

3MT-N700X

CCAGTGSNNAGAACGTCTGTTAG

5MT-H701X

AGACGTTCTAACNNSTGGATGGGTAC

3MT-H701X

GTACCCATCCASNNGTTAGAACGTCT

5MT-N700S

CAGACGTTCTTCTCACTGGATGGGTAC

3MT-N700S

AGAACGTCTGTTAGAAGGAGAC