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Table 2 Apparent kinetic constants of r CDH and variant N700S

From: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

  

Cellobiose

Electron acceptor

Enzyme

Km(μM)

kcat(s-1)

kcat/Km(M-1 s-1)

DCIP

r CDH

11.4 ± 0.7

4.1 ± 0.1

360000

 

N700S

33 ± 1

12.4 ± 0.6

375000

1,4-benzoquinone

r CDH

22 ± 1

4.3 ± 0.1

185000

 

N700S

56 ± 2

13.4 ± 0.5

240000

oxygen

r CDH

37 ± 3

0.042 ± 0.003

1100

 

N700S

73 ± 6

0.19 ± 0.01

2600

  1. Kinetic constants for cellobiose were determined with DCIP, 1,4-benzoquinone and oxygen as electron acceptors at pH 6.0 and 30°C.
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Microbial Cell Factories

ISSN: 1475-2859

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