Table 1 Purification scheme of CDH variant N700S
From: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U mg-1) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Culture supernatant | 7380 | 3360 | 2.2 | 100 | 1 |
Phenyl-Sepharose | 5980 | 950 | 6.3 | 81 | 2.9 |
Q-Source | 5540 | 690 | 8.0 | 75 | 3.6 |