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Table 1 Genotypes and phenotypes of P.pastoris, Isolates and Strains used

From: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter

P. P.pastoris

P.pastoris strain/

Colony

Genotype his/met

Exported protein phenotype Or

 

Host strain -

Isolate- colony derived

Designations

 

Methanol utilization

Quantified

 

Source

   

(MutSor Mut+)

(see Figures3,4,5)

1

GS115

host

h

his4, Mut+

His-, Mut+

UP1, UP3

2

GS115

vector-Host

v-H

aox1::pPIC9K, HIS4, Mut S

His+, Muts

UP1, UP3

3

GS115

β-Galactosidase

β-G or (β-Gal)

his4::pPIC9K-lacZ, HIS4, Mut +

His+, Mut+ β-gal

-

4

GS115

Human Serun Albumin

H or (HSAsec)

aox1::pPIC9K-HSA, HIS4, Mut S

His+, Muts, HSA

HSA, UP1, UP3

5

GS115

E. coli Alk. Phos.(Parental)

E(P)

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

6

GS115

E. coli Alk. Phos.(PCR-Mut.)

E(D2)

aox1::pPIC9-phoA (D2,TrlStop), HIS4, Mut S

His+, Muts, phoA-

-

7

GS115

E. coli Alk. Phos.(PCR-Mut.)

E(F2)

aox1::pPIC9-phoA (F2,TrlStop), HIS4, Mut S

His+, Muts, phoA-

-

8

GS115

E. coli Alk Phos.(Mutant)

E(M9)-A

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

9

GS115

E. coli Alk. Phos.(Mutant)

E(M9)-B

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

10

GS115

E. coli Alk. Phos.(Mutant)

E(M32)-A

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

11

GS115

E. coli Alk. Phos.(Mutant)

E(M32)-B

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

12

GS115

E. coli Alk. Phos.(Mutant)

E(M32)-C

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

13

GS115

E. coli Alk. Phos.(Mutant)

E(M32)-D

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

14

GS115

E. coli Alk. Phos.(Mutant)

E(M44)-A

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3

15

GS115

E. coli Alk. Phos.(Mutant)

E(M44)-B

aox1::pPIC9-phoA (A3#27), HIS4, Mut S

His+, Muts, phoA+

α-sec-EAP, UP1, UP3