Figure 4From: A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporterLevels of over-secretion of α-sec-EAP, UP1 and UP3 can vary independently with E(M). Values or mean values of ROIs corresponding to α-sec-EAP and HSA (42 and 69 hours inductions) from Western Blot Analysis normalized to E(P) 69 hours (Figure 3) and Gel Code Blue stained gels from 170 hour inductions normalized to UP3 from E(P) (Figure 5) were plotted as semi-log scales (Figure 4a, graphs WB and GCB). Plotted values were taken from tables 2, 3 and 4. However, to permit graphical resolution of low values, results of HSA secreted after 69 hours and 170 hours of induction were omitted from the plots. Mean values of UP1 and UP3 expressed as their ratios in E(P)/E(P), v-H/E(P) and all E(M)/E(P) were plotted (Figure 4b).Back to article page