Thermal adaptability of Kluyveromyces marxianus in recombinant protein production

Table 1 Effects of the temperature on growth of K. marxianus L3 wild-type and recombinant strains

T(°C)	Wild-type	Wild-type	Wild-type	rHSA	GAA	KlSod1p
T(°C)	DW (gL^-1)	Q_x(gL^-1 h^-1)	μ (h^-1)	μ (h^-1)	μ (h^-1)	μ (h^-1)
5	10.5^#	0.096	0.041	0.034^a	0.031^a	0.034^a
15	12.8^‡	0.355	0.171	0.138^a	0.160	0.144^a
30	12.9^‡	0.479	0.377	0.321^a	0.341^a	0.271
40	10.6^#	0.443^‡	0.439	0.293^a	0.369	0.313^a
45	9.8	0.426^‡	0.464	-	-	-

The strains were cultured at different temperatures in YPD medium containing 20 g L^-1 glucose. The specific growth rates (μ), the biomass yield (expressed as dry weight grams per litre of culture) and the biomass volumetric productivities (Q_X) are reported as a function of growth temperature. The values are means of three independent experiments, with standard deviations always lower than 5%. Superscripts within a column (symbols) and within a row (letters) indicate statistically similar means, P > 0.05.

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