Figure 4

Effects of the growth temperature on Sodp secretion. Culture samples, grown at 5 - 40°C, have been analyzed both at the growth and stationary phases. A) extracellular SOD activity measured by zymogram analysis; B) northern blot analysis of SOD1 mRNA. Data are means of three independent experiments ± standard deviations. The values, means of three independent experiments ± standard deviations, have been normalized setting at 1 the highest value. Superscripts indicate statistically similar means (P > 0.05). Symbols are used to compare sample grown at the same temperature, collected at different growth phases (exponential or stationary). Letters are used to compare samples collected in the same growth phase, cultured at different temperatures.