New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure

Table 2 Overview of the most relevant cellular functions affected by elevated pressure alone and by elevated pressure and DOT

Cellular function	Differentially expressed genes
	*Pressure*	*Pressure oxygen*
Stress sensing/stress response	• aer-1 (-), MCP (-)	• aer-1 (-), MCP (-)
	• rpoH and heat-shock genes (+)	• rpoH and heat-shock genes (+)
	• DNA repair (-)	• DNA repair (-)
Control of reactive oxygen species	• ahpC, glutathione genes (+)	• ahpC, glutathione genes (+)
	• anr (+) and cyo (+)	• anr (-) and cyo (+)
	• Cbb₃-1 (+), Cbb₃-2 (-), Aa₃ (-), Cyo (+), azu (+)	• Cbb₃-1 (-), Cbb₃-2 (-), Aa₃ (-), Cyo (+), azu (-)
Cell envelope	• oprG (+), oprH (+)	• oprG (-), oprH (+)
	• membrane proteins	• membrane proteins
	• secretion systems (+)	• secretion systems (+)
Internal pH	• urease (+)	• secretion systems (+)
	• secretion systems (+)
	• oxidative stress (+)	• oxidative stress (+)
Fe homeostasis/Fe-S clusters	• siderophore transporters (+), bacterioferritin (-)
		• siderophore transporters (+), bacterioferritin (-)
Biosynthetic metabolism		• ADI (-)
		• Glu synthesis/metabolism (+)

Induction and repression of gene transcription is indicated by the signs (+) and (-), respectively, and major differences of gene transcription between the two conditions are indicated by the bold font. Abbreviations: ADI, arginine deiminase pathway; Glu, glutamate; MCP, methyl-accepting chemotaxis protein.

ISSN: 1475-2859