| |
WT
|
TEFmut7
|
TEFmut2
|
|---|
|
Fermentation time (h)
|
70
|
146
|
167
|
|
Growth time (h)
|
24
|
60
|
133
|
|
μmax (h-1)
|
0.29 ± 0.01
|
0.13 ± 0.01
|
0.046 ± 0.001
|
|
DCWmax (g L-1)
|
12.0 ± 0.5
|
8.0 ± 0.5
|
3.8 ± 0.2
|
|
Y DCW/glucose g g-1
|
0.076 ± 0.001
|
0.074 ± 0.003
|
0.055 ± 0.001
|
|
[0–16 h]
|
[0–30 h]
|
[0–44 h]
|
|
Y glycerol/glucose g g-1
|
0.056 ± 0.001
|
0.031 ± 0.003
|
0.022 ± 0.004
|
|
[0–16 h]
|
[0–30 h]
|
[0–44 h]
|
|
Y glycerol/DCW g g-1
|
0.74 ± 0.03
|
0.42 ± 0.02
|
0.40 ± 0.04
|
|
[0–16 h]
|
[0–30 h]
|
[0–44 h]
|
|
Y acetate/ glucose g g-1
|
0.005 ± 0.001
|
n.d.
|
n.d.
|
|
Y pyruvate/ glucose g g-1
|
0.003 ± 0.001
|
n.d.
|
n.d.
|
|
Y succinate/ glucose g g-1
|
0.004 ± 0.001
|
0.013 ± 0.001
|
0.026 ± 0.001
|
|
Yethanol/glucose g g-1 (growth)
|
0.45 ± 0.003
|
0.46 ± 0.008
|
0.48 ± 0.005
|
|
Yethanol/glucose g g-1 (overall)
|
0.47 ± 0.001
|
0.48 ± 0.003
|
0.48 ± 0.005
|
|
[ethanol] final (g L-1)
|
139 ± 1
|
106.0 ± 0.5
|
57 ± 2
|
|
[glycerol] final (g L-1)
|
9.0 ± 0.3
|
5.4 ± 0.1
|
2.8 ± 0.2
|
|
Ethanol productivity (g L-1 h-1)
|
1.97 ± 0.10
|
0.73 ± 0.03
|
0.33 ± 0.02
|
|
Pcritical (g L-1)
|
90 ± 2
|
78 ± 1
|
54 ± 2
|
- μmax: maximum specific growth rate, DCWmax maximum cell concentration , Yi/j yield of production of constituent i on the constituent j: DCW biomass ;.n.d. : not detected (detection thresholds were 60, 50 and 100 mg L-1 for acetate, pyruvate and succinate respectively). Yields, concentrations, and specific rates are average values of 2 independent fermentations. Productivity and fermentation time correspond to the experiments shown in the paper.
