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Table 2 Strains and plasmids used in this study

From: Expression and functional studies of genes involved in transport and metabolism of glycerol in Pachysolen tannophilus

Plasmid or strain

Relevant characteristics

Source or reference

Plasmids

  

PUG6

Plasmid with loxP-kanMX-loxP disruption cassette

[23]

pSP-G1

2μ-based URA3 plasmid with TEF1/PGK1 promoter

[25]

pXI-5

Integrative USER vector, with reusable URA3 Marker

[24]

pSH47

Centromeric plasmid, URA3, PGAL1-Cre- TCYC1

[23]

pXI-5-PtFPS1

pXI-5 with TEF1/PGK1 promoter and PtFPS1 gene

This study

pXI-5-PtFPS2

pXI-5 with TEF1/PGK1 promoter and PtFPS2 gene

This study

pXI-5-PtSTL1

pXI-5 with TEF1/PGK1 promoter and PtSTL1 gene

This study

pXI-5-PtSTL2

pXI-5 with TEF1/PGK1 promoter and PtSTL2 gene

This study

pXI-5-ScFPS1

pXI-5 with TEF1/PGK1 promoter and ScFPS1 gene

This study

pXI-5- ScSTL1

pXI-5 with TEF1/PGK1 promoter and ScSTL1 gene

This study

S.cerevisiae Strains

  

CEN.PK 113-5D

MAT a MAL2-8 c SUC2 ura3-52

Peter Kötter

stl1Δ

CEN.PK 113-5D Stl1 Δ (490,1279 )::loxP

This study

PtFPS1_XY1

stl1Δ with plasmid pXI-5-PtFPS1

This study

PtFPS2_XY2

stl1Δ with plasmid pXI-5-PtFPS2

This study

PtSTL1_XY3

stl1Δ with plasmid pXI-5-PtSTL1

This study

PtSTL2_XY4

stl1Δ with plasmid pXI-5-PtSTL2

This study

ScFPS1_XY5

stl1Δ with plasmid pXI-5-ScFPS1

This study

ScSTL1_XY6

stl1Δ with plasmid pXI-5- ScSTL1

This study