Plasmid or strain | Relevant characteristics | Source or reference |
---|---|---|
Plasmids | Â | Â |
PUG6 | Plasmid with loxP-kanMX-loxP disruption cassette | [23] |
pSP-G1 | 2μ-based URA3 plasmid with TEF1/PGK1 promoter | [25] |
pXI-5 | Integrative USER vector, with reusable URA3 Marker | [24] |
pSH47 | Centromeric plasmid, URA3, PGAL1-Cre- TCYC1 | [23] |
pXI-5-PtFPS1 | pXI-5 with TEF1/PGK1 promoter and PtFPS1 gene | This study |
pXI-5-PtFPS2 | pXI-5 with TEF1/PGK1 promoter and PtFPS2 gene | This study |
pXI-5-PtSTL1 | pXI-5 with TEF1/PGK1 promoter and PtSTL1 gene | This study |
pXI-5-PtSTL2 | pXI-5 with TEF1/PGK1 promoter and PtSTL2 gene | This study |
pXI-5-ScFPS1 | pXI-5 with TEF1/PGK1 promoter and ScFPS1 gene | This study |
pXI-5- ScSTL1 | pXI-5 with TEF1/PGK1 promoter and ScSTL1 gene | This study |
S.cerevisiae Strains | Â | Â |
CEN.PK 113-5D | MAT a MAL2-8 c SUC2 ura3-52 | Peter Kötter |
stl1Δ | CEN.PK 113-5D Stl1 Δ (490,1279 )::loxP | This study |
PtFPS1_XY1 | stl1Δ with plasmid pXI-5-PtFPS1 | This study |
PtFPS2_XY2 | stl1Δ with plasmid pXI-5-PtFPS2 | This study |
PtSTL1_XY3 | stl1Δ with plasmid pXI-5-PtSTL1 | This study |
PtSTL2_XY4 | stl1Δ with plasmid pXI-5-PtSTL2 | This study |
ScFPS1_XY5 | stl1Δ with plasmid pXI-5-ScFPS1 | This study |
ScSTL1_XY6 | stl1Δ with plasmid pXI-5- ScSTL1 | This study |