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Table 2 Strains and plasmids used in this study

From: Expression and functional studies of genes involved in transport and metabolism of glycerol in Pachysolen tannophilus

Plasmid or strain Relevant characteristics Source or reference
Plasmids   
PUG6 Plasmid with loxP-kanMX-loxP disruption cassette [23]
pSP-G1 2μ-based URA3 plasmid with TEF1/PGK1 promoter [25]
pXI-5 Integrative USER vector, with reusable URA3 Marker [24]
pSH47 Centromeric plasmid, URA3, PGAL1-Cre- TCYC1 [23]
pXI-5-PtFPS1 pXI-5 with TEF1/PGK1 promoter and PtFPS1 gene This study
pXI-5-PtFPS2 pXI-5 with TEF1/PGK1 promoter and PtFPS2 gene This study
pXI-5-PtSTL1 pXI-5 with TEF1/PGK1 promoter and PtSTL1 gene This study
pXI-5-PtSTL2 pXI-5 with TEF1/PGK1 promoter and PtSTL2 gene This study
pXI-5-ScFPS1 pXI-5 with TEF1/PGK1 promoter and ScFPS1 gene This study
pXI-5- ScSTL1 pXI-5 with TEF1/PGK1 promoter and ScSTL1 gene This study
S.cerevisiae Strains   
CEN.PK 113-5D MAT a MAL2-8 c SUC2 ura3-52 Peter Kötter
stl1Δ CEN.PK 113-5D Stl1 Δ (490,1279 )::loxP This study
PtFPS1_XY1 stl1Δ with plasmid pXI-5-PtFPS1 This study
PtFPS2_XY2 stl1Δ with plasmid pXI-5-PtFPS2 This study
PtSTL1_XY3 stl1Δ with plasmid pXI-5-PtSTL1 This study
PtSTL2_XY4 stl1Δ with plasmid pXI-5-PtSTL2 This study
ScFPS1_XY5 stl1Δ with plasmid pXI-5-ScFPS1 This study
ScSTL1_XY6 stl1Δ with plasmid pXI-5- ScSTL1 This study