Skip to main content

Table 4 Oligonucleotides used in this study

From: A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

Name

Sequence (5′→ 3′)

a) PCR primers

 

Pwitw4_AscI

AAAGTGAGGGCGCGCCGGTTGATGAGAG

Pwitw5_SpeI

ATCCACCGGAACTAGTCCCCTGCTC

Pwitw6_badF

AGACTAGTAAGCCCTCCCGTATCGTAGTTA

Pwitw6_badR

TGGCGCGCCAGATGCGTAAGGAGAAAATACCG

ET_AgeI_fwd

GATGGCCCATATGATATCTCCTTCT

ET_NdeI_rev

GATCACCGGTCCAGTGATCGAA

BAD_BbsI_fwd

GGCCTTTCGTCTTCCCGGCATCCGCTTACAGACA

BAD_NdeI_rev

GACGCCCATATGTAATTCCTCCTGTTAGCCCAAAAAACG

TRC_AgeI_fwd1

TGCATGTGTCACCGGTTTTCACCGTC

TRC_NdeI_rev1

GAGCTCGAATCATATGGTCTGTTTCCTG

pelB_fwd

AGCTACATATGAAATACCTATTGCCTACG

APhis_rev2

AGGATCCGAGCCTTTCGTTTTATTGATGC

b) qRT-PCR primers

 

RT-synluc_fwd2

CCATGGCTTCGGCATGTT

RT-synluc_rev2

ACACGAAAGCCGCAAATCA

gfpmut3_fwd1

CATGGCCAACACTTGTCACT

gfpmut3_rev1

CTGCTTCATGTGATCTGGGTATCT

RT-hGH.fwd1

GCCTGTGTTTTAGCGAAAGCAT

RT-hGH.rev1

AGATTGCTTTTCTGCTGGGTTT

RT-IL-1-RA.fwd1

ATTGATGTGGTGCCGATTGA

RT-IL-1-RA.rev1

TCAGACACATTTTACCACCATGAA

scFv198.fwd

GAAGGGCCGGTTCACCAT

scFv255.rev

CATTTGCAGATACAGCGTGTTCT

RT-16S-Fwd

ATTGACGTTACCCGCAGAAGAA

RT-16S-Rev

GCTTGCACCCTCCGTATTACC