Table 1 Plasmids used in this study ^a

pTA16/pSB-M1b^b

m-toluate- inducible Pm, xylS activator gene, RK2 replicon, bla reporter, Kan^r

[31]

pET16b

IPTG-inducible P _T7lac , lacI repressor gene, Amp^r

Novagen

pBAD/gIII_calmodulin

L-arabinose- inducible P _BAD , araC activator gene, Amp^r

Invitrogen

pTrc99A

IPTG- inducible P _trc promoter, lacI repressor gene, Amp^r

Pharmacia

pIB11-luc_S

pIB11 [25] with luc _S under control of xylS/Pm, Kan^r

unpublished

pBAD24-GFP

pBAD24 with gfpmut3 insert, Amp^r

[32]

pHOG-173-2-5-AP

pHOG plasmid with scFv173-2-5-phoA fusion gene insert, provided by Affitech AS, Oslo, Amp^r

unpublished

pMA-GH

pMA vector (GeneArt®, Invitrogen) with GH1 _S insert, provided by Vectron Biosolutions AS, Trondheim, Amp^r

unpublished

pMA-T-IL-1RA

pMA vector (GeneArt®, Invitrogen) with IL1RN _S insert, provided by Vectron Biosolutions AS, Trondheim, Amp^r

unpublished

pSB-P0x

pSB-M1b variants with combinations of different features:

P… regulator/promoter system

M… xylS/P m

M-1-17… xylS/P m variant ML1-17

E… lacI/P _T7lac (from pE T)

T… lacI^q/P _trc (from pT rc)

B… araC/P _BAD (pB AD)

0… origin of replication

1… RK2 replicon

2… pMB1 replicon

x… reporter gene

b… bla

l… luc _S

s… scFv173-2-5-phoA

g… gfpmut3

h… GH1 _S

r… IL1RN _S

This study

e.g. pSB-M2l

m-toluate- inducible P _m , xylS activator gene, pMB1 ori, luc _S reporter, Kan^r

This study