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Figure 4 | Microbial Cell Factories

Figure 4

From: A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

Figure 4

Investigation of the tightness of different regulator/promoter systems in combination with the pMB1 replicon. Protein activity was determined in parallel with induced cultures at the time point corresponding to five hours after induction. The data presented are from independet biological replica. The following E. coli strains were used as expression hosts. Panels A-C: ER2566. Panels D-E: DH10B. The capital letters represent the regulator/ promoter systems according to Table 1. Uninduced expression of GFP in DH10B was very close to the detection limit in LB medium and was left out.

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Microbial Cell Factories

ISSN: 1475-2859

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