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Figure 2 | Microbial Cell Factories

Figure 2

From: A comparative analysis of the properties of regulated promoter systems commonly used for recombinant gene expression in Escherichia coli

Figure 2

Maximum expression of three different genes placed under control of different regulator/promoter systems. Data represent relative expression levels under induced conditions where the activity of M1x (gene x under conrol of the Pm wildtype promoter, RK2 replicon) was set to 1.0. Expression was induced in a way that activity levels were maximized: 2 mM m-toluate for strains harboring XylS/Pm- based constructs, 1 mM IPTG for those with LacI/PT7lac, 0.2 mM IPTG for LacI/Ptrc and 0.015% L-arabinose for AraC/PBAD. The following E. coli strains were used as expression hosts. Panels A-C: ER2566. Panels D-F: DH10B. The naming code is the following: The capital letter represents the regulator/promoter system and the digit represents the origin of replication; for details see Table 1. The data presented are from independent biological replica.

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Microbial Cell Factories

ISSN: 1475-2859

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