Table 1 Oligonucleotide list
Name | Sequence (5’-3’) | Restriction site |
|---|---|---|
cotY-F | GCT TAG GAT CC A TGA TGA TGT GTA CGA TTG | Bam HI |
cotZ-R | CGT AGC GAA TTC AGT TAT CAC TCT TGT CCT C | Eco RI |
Linker-gggs-F | CCG GAA TTC ACG GAT TAG GCC GTT TGT CCT CAT GGA CCC GTA TAA AAA GAA TGA TAT TGA GCG TTT TGA CCG TGA G | Eco RI |
Linker-gggs-R | CGC GAG CTC TGC GCG GCC GCT AGA GCC ACC GCC ACC GTA GGA TCC GGA TGA TTG ATC ATC TGA AGA TTT TAG TGA TCG TTT AGA TG | SacI |
linker-gggeaaakggg-F | GCC TGT TAG GAA TTC CGC TCC AAT CTC TTT TTA CAA TAG AAT ATA TGG AAC CGA AAA TCA TGG CGA TGT ATG AAC GGA TTA GGC C | Eco RI |
linker- gggeaaakggg- R | CGC GGA TCC TCC TCC ACC TTT CGC TGC TGC TTC TCC TCC ACC GGA TGA TTG ATC ATC TGA AG | Bam HI |
ureA-F | GAG GGA TCC ATG AAA CTC ACC CCA AAA G | Bam HI |
ureA-R | CGC GAG CTC TAG GGC CAT ACA TAG AAA C | SacI |