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Figure 3 | Microbial Cell Factories

Figure 3

From: New stable anchor protein and peptide linker suitable for successful spore surface display in B. subtilis

Figure 3

Localisation of CotZ-UreA and CotB-GGGEAAAKGGG-UreA fusion proteins as assessed by immunofluorescence microscopy. Purified, free spores of wild type strain 168, BKH102 (cotZ-ureA) and BKH124 (cotB- GGGEAAAKGG-ureA) were visualised by phase contrast (PC) and immunofluorescence (IF) microscopy. The spores were incubated with mouse anti-UreA antibodies, followed by anti-mouse IgG-Cy3 conjugates. The same exposure time was used for IF images.

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Microbial Cell Factories

ISSN: 1475-2859

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