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Figure 1 | Microbial Cell Factories

Figure 1

From: New stable anchor protein and peptide linker suitable for successful spore surface display in B. subtilis

Figure 1

Schematic steps of building strains carrying fusion genes. A fragment containing the operon cotYZ was amplified and cloned into the pDL vector. Next, fragment containing the entire ureA gene was amplified and cloned in frame with the cotZ gene resulting in plasmid pKH102 (panel A). As a result we obtained the fusion protein CotZ-UreA (panel B). Similarly the CotB-GGGGS-UreA (panel C) and CotB-GGGEAAAKGGG-UreA (panel D) fusions were obtained. All numbers indicate amino acid residues. Next, pKH102 after linearization is integrated into the B. subtilis chromosome after a double crossing-over event (panel E), generating strain BKH102, which contains the cotZ-ureA fusion gene under control of the sigma K promoter.

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Microbial Cell Factories

ISSN: 1475-2859

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