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Figure 3 | Microbial Cell Factories

Figure 3

From: Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation

Figure 3

Effect of temperature on biomass and SELP production. Comparison of maximum SELP-59-A volumetric (blue) and specific (grey) productivities as well as maximum biomass levels measured (red) as a function of the incubation temperature used. The right hand side of the figure (25–30, 25–37, 30–37, 37–30, 37–42°C) represents the temperature shift experiments: the initial temperatures used and the temperatures after induction. The maximum biomass values represent the highest dry cell weights (DCW, g/L) measured over the course of the experiment. The SELP-59-A volumetric and specific productivities were estimated from comparisons of band intensities on a sodium dodecyl sulphate polyacrylamide gel with a band of known concentration.

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