Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Manabe, Kenji; Kageyama, Yasushi; Morimoto, Takuya; Shimizu, Eri; Takahashi, Hiroki; Kanaya, Shigehiko; Ara, Katsutoshi; Ozaki, Katsuya; Ogasawara, Naotake

doi:10.1186/1475-2859-12-18

Table 2 Genes down-regulated in 874ΔrocG cells (bottom-ranked 20 genes)

From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Gene^a	Product^a	Function^a	Average signal^b		Ratio^c	Transcriptional factor^d
			MGB874	874ΔrocG
yuiA	unknown	unknown	4307	533	0.12
yycC	unknown	unknown	1915	273	0.14	TnrA(−)
yycB	unknown	unknown	1883	287	0.15	TnrA(−)
dhbC	isochorismate synthase	biosynthesis of the siderophore bacillibactin	2560	529	0.21	Fur(−)
pel	pectate lyase C	degradation of polygalacturonic acid	3736	808	0.22	ComA(+), TnrA(−)
ilvB	acetolactate synthase (large subunit)	biosynthesis of branched-chain amino acids	2106	459	0.22	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
leuB	3-isopropylmalate dehydrogenase	biosynthesis of leucine	2072	467	0.23	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
dhbF	unknown	biosynthesis of the siderophore bacillibactin	2081	474	0.23	Fur(−)
serA	phosphoglycerate dehydrogenase	biosynthesis of serine	2575	603	0.23
leuA	2-isopropylmalate synthase	biosynthesis of leucine	1680	396	0.24	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
dhbB	isochorismatase	biosynthesis of the siderophore bacillibactin	1897	447	0.24	Fur(−)
leuC	3-isopropylmalate dehydratase (large subunit)	biosynthesis of leucine	2166	516	0.24	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
ilvC	ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate)	biosynthesis of branched-chain amino acids	2981	713	0.24	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
yocS	putative sodium-dependent transporter	unknown	1028	248	0.24
yodF	unknown	unknown	737	182	0.25	TnrA(−)
ydzA	unknown	unknown	1035	266	0.26
yxxG	unknown	unknown	435	119	0.27	DegU(−), YvrH(+)
dhbE	2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E	biosynthesis of the siderophore bacillibactin	1539	439	0.29	Fur(−)
leuD	3-isopropylmalate dehydratase (small subunit)	biosynthesis of leucine	1137	327	0.29	CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
yuiB	unknown	unknown	3785	1150	0.3

^aThe SubtiWiki was used as a reference for the genes, products and functions [21].
^bThe average signal intensities of probes in each coding sequence.
^cThe ratio of each of the genes was obtained by dividing the average signal intensity in each coding sequence of 874ΔrocG cells by that for MGB874 cells.
^dThe Database of Transcriptional Regulation in Bacillus subtilis (DBTBS) was used as a reference [22]. Transcriptional activators or repressors are indicated by a (+) or (−), respectively. TnrA is shown in bold type.

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ISSN: 1475-2859

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