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Table 2 Genes down-regulated in 874ΔrocG cells (bottom-ranked 20 genes)

From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditions

Genea Producta Functiona Average signalb Ratioc Transcriptional factord
    MGB874 874ΔrocG   
yuiA unknown unknown 4307 533 0.12  
yycC unknown unknown 1915 273 0.14 TnrA(−)
yycB unknown unknown 1883 287 0.15 TnrA(−)
dhbC isochorismate synthase biosynthesis of the siderophore bacillibactin 2560 529 0.21 Fur(−)
pel pectate lyase C degradation of polygalacturonic acid 3736 808 0.22 ComA(+), TnrA(−)
ilvB acetolactate synthase (large subunit) biosynthesis of branched-chain amino acids 2106 459 0.22 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
leuB 3-isopropylmalate dehydrogenase biosynthesis of leucine 2072 467 0.23 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
dhbF unknown biosynthesis of the siderophore bacillibactin 2081 474 0.23 Fur(−)
serA phosphoglycerate dehydrogenase biosynthesis of serine 2575 603 0.23  
leuA 2-isopropylmalate synthase biosynthesis of leucine 1680 396 0.24 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
dhbB isochorismatase biosynthesis of the siderophore bacillibactin 1897 447 0.24 Fur(−)
leuC 3-isopropylmalate dehydratase (large subunit) biosynthesis of leucine 2166 516 0.24 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
ilvC ketol-acid reductoisomerase (2,3-dihydroxy-3-methylbutanoate, 2-acetolactate) biosynthesis of branched-chain amino acids 2981 713 0.24 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
yocS putative sodium-dependent transporter unknown 1028 248 0.24  
yodF unknown unknown 737 182 0.25 TnrA(−)
ydzA unknown unknown 1035 266 0.26  
yxxG unknown unknown 435 119 0.27 DegU(−), YvrH(+)
dhbE 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E biosynthesis of the siderophore bacillibactin 1539 439 0.29 Fur(−)
leuD 3-isopropylmalate dehydratase (small subunit) biosynthesis of leucine 1137 327 0.29 CcpA(+), CodY(−), TnrA(−), TrnS-Leu2(+)
yuiB unknown unknown 3785 1150 0.3  
  1. aThe SubtiWiki was used as a reference for the genes, products and functions [21].
  2. bThe average signal intensities of probes in each coding sequence.
  3. cThe ratio of each of the genes was obtained by dividing the average signal intensity in each coding sequence of 874ΔrocG cells by that for MGB874 cells.
  4. dThe Database of Transcriptional Regulation in Bacillus subtilis (DBTBS) was used as a reference [22]. Transcriptional activators or repressors are indicated by a (+) or (−), respectively. TnrA is shown in bold type.