Figure 5From: Improved production of secreted heterologous enzyme in Bacillus subtilisstrain MGB874 via modification of glutamate metabolism and growth conditionsTranscriptional levels of selected genes in strains MGB874 and 874ΔrocG. The strains MGB874 (A) and 874∆rocG (B) were transformed with pHYS237, and cultured by the pH-Stat fermentation. Fermentation without pH control was done as a reference (a). The pH was adjusted to 7.2 by addition of NaOH (b) or aqueous NH3 (c). RNA was isolated from the cells at the 24 h of the cultivation time (indicated by arrows in Figure 4), and expression level of htrB, nrgA, gltA and citB were determined by qRT-PCR. The transcriptional levels are expressed relative to those of 16S rRNA. Error bars represent standard deviations (n=3).Back to article page