Figure 1

The reduction of hydrophobic interactions between the conjugated GFP-containing IBs (gfpIBs) and the SabA adhesin aggregated to IBs (sabIBs). A - gfpIBs conjugated with albumin using nonpolar glycine for glutaraldehyde inactivation. B - gfpIBs conjugated with albumin using polar lysine for glutaraldehyde inactivation. Conjugates gfpIBs-albumin (1.25 mg of gfpIBs in 400 μl of PBS) were 1.5-fold diluted from line H to D in 10 μl of PBS. The suspension of sabIBs (isolated from 10 mg of lyophilized transformed E. coli in 1000 μl of PBS, diluted in ratio 1:16) was 1.5- (A) or 1.2-fold (B) serial diluted from column 1 to 12 in 15 μl of PBS. Finally, the wells were titrated with 50 μl of the RBC suspension. Line A - negative control (RBC in PBS), Line B (green frame) - interaction of sabIBs with nonconjugated gfpIBs and RBC, Line C - control interaction of sabIBs with RBC. Blue frame defines the zone of positive hemagglutination.