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Table 2 Protein quantification by whole cell fluorescence measurements

From: Nitrogen catabolite repressible GAP1 promoter, a new tool for efficient recombinant protein production in S. cerevisiae

Protein Expected size (kDa) Fluorescence * Estimated quantity
(Arbitrary units) (μg per g of cells**)
24 h. 48 h. 24 h. 48 h.
Gap1-GFP/10His 95.2 3325 ± 49 1971 ± 54 100 60
N-ter Gap1-GFP/10His 38.7 4424 ± 18 733 ± 38 19 18
Uga4-GFP/10His 91.2 16801 ± 514 17914 ± 123 476 508
Vglut1-GFP/10His 90.9 8032 ± 161 3129 ± 32 226 88
MD-2-GFP/10His 47.6 865 ± 113 4130 ± 66 7 6
  1. *Background signal from non induced cells was subtracted.
  2. **Wet weight.