Nitrogen catabolite repressible GAP1 promoter, a new tool for efficient recombinant protein production in S. cerevisiae

Table 2 Protein quantification by whole cell fluorescence measurements

Protein	Expected size (kDa)	Fluorescence *		Estimated quantity
		(Arbitrary units)		(μg per g of cells**)
		24 h.	48 h.	24 h.	48 h.
Gap1-GFP/10His	95.2	3325 ± 49	1971 ± 54	100	60
N-ter Gap1-GFP/10His	38.7	4424 ± 18	733 ± 38	19	18
Uga4-GFP/10His	91.2	16801 ± 514	17914 ± 123	476	508
Vglut1-GFP/10His	90.9	8032 ± 161	3129 ± 32	226	88
MD-2-GFP/10His	47.6	865 ± 113	4130 ± 66	7	6

ISSN: 1475-2859