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Figure 3 | Microbial Cell Factories

Figure 3

From: A moth pheromone brewery: production of (Z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory

Figure 3

Expression and co-expression of AseΔ11 and AseFAR in S. cerevisiae. A) Alcohol products from AseFAR expression (top) supplemented with Z11-16:ME, compared to vector only negative control (bottom). AseFAR produces the corresponding Z11-16:OH, as well as 12:OH, 14:OH, 16:OH, and Z9-16:OH, as a result from conversion of natural FA substrates present in yeast. None of these alcohols are found in the negative control. B) DMDS derivatization of Ase∆11-expressing yeast subjected to base methanolysis (top) compared to an empty vector control (bottom). The Ase∆11 chromatogram shows peaks corresponding to ∆11-12:DMDS, Z11-14:DMDS, E11-14:DMDS, and Z11-15:DMDS, which are not present in the negative control. Additionally, there is a substantially higher peak in AseΔ11 corresponding to Z11-16:DMDS (also see additional file 1). C) Base methanolysed yeast extract from Ase∆11/CUP1p-AseFAR/GAL1p-pYEXCHT (top panel) as compared to a negative control (bottom panel) showing the presence of Z11-16:ME in the former but not the latter. D) Alcohol extracts from AseFAR-pYES2.1 (top panel) compared with Ase∆11/CUP1p-AseFAR/GAL1p-pYEXCHT (bottom panel). Both samples contain 12:OH, 14:OH, 16:OH, and Z9-16:OH, but only the latter contains Z11-16:OH.

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Microbial Cell Factories

ISSN: 1475-2859

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