Table 3 E. coli strains and plasmid used in this study

DH5α

F^–, ø80dlacZ ΔM15, Δ(lacZYA-argF)U169, deo R, recA 1, endA 1, hsdR 17(rK^–, mK⁺), gln V44, supE 44, λ^-, thi-1, gyrA 96, relA 1, nup G

[27]

JM109

endA 1, glnV 44, thi-1, relA 1, gyrA 96, recA 1, mcrB⁺, Δ(lac-proAB), e14-, [F’ traD 36, proAB⁺, lacI^q, lacZ ΔM15], hsdR 17(r_K^-m_K⁺)

[28]

XL-1 Blue

endA 1, gyrA 96(nal^R), thi-1, recA 1, relA 1, lac, glnV 44, F’[ ::Tn10, proAB⁺, lacI^q, Δ(lacZ)M15], hsdR 17(r_K^- m_K⁺)

[29]

S17-1

tmpR, spcR, strR, recA pro hsdR RP4-2-Tc::Mu-Km::Tn7

[30]

W3110

F,^-λ^-, rph-1, INV(rrnD, rrnE)

[31]

BL21(DE3)

F ^- , ompT, gal, dcm, lon, hsdS _B (r _B ^- m _B ^- ), λ(DE3), [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

[32]

Plasmid

pKSSE5.3

phaC, orfZ, Ampr

[16]