Poly(4-hydroxybutyrate) (P4HB) production in recombinant Escherichia coli: P4HB synthesis is uncoupled with cell growth

Table 2 Comparison of carbon sources for growth and P4HB accumulation of E. coli JM109 (pKSSE5.3)

Carbon source	Xylose	Glucose	Glycerol
OD₆₀₀	3.4 ± 1.4	3.9 ± 1.1	7.6 ± 0.4
CDW (g L^-1)	2.16 ± 0.37	2.04 ± 0.60	3.80 ± 0.18
μ (h^-1)	0.32 ± 0.09	0.38 ± 0.04	0.35 ± 0.01
P4HB content% (w w^-1)	32 ± 3.7	19 ± 6.4	12 ± 3.6
P4HB concentration (g L^-1)	0.65 ± 0.11	0.36 ± 0.05	0.41 ± 0.00

The cells were cultivated in a 1 L bioreactors at 37°C with an agitation of 500 rpm in modified E2 minimal medium supplemented with 4 g L^-1 Na-4HB, 1 g L^-1 NZ-amines, 100 μg mL^-1 ampicillin and 0.015 g L^-1 thiamine. Xylose, glucose or glycerol was used as the growth substrate. The growth was followed with time and samples were taken after 25 h for P4HB analysis. The data were obtained from two independent cultivations.

ISSN: 1475-2859