Figure 2

Influence of temperature on the growth and P4HB accumulation of E. coli JM109 (pKSSE5.3). The cells were grown in modified E2 minimal medium supplemented with 10 g L^-1 xylose, 4 g L ^-1 Na-4HB, 1 g L^-1 NZ-amines, 100 μg mL^-1 ampicillin and 0.015 g L^-1 thiamine. Four different temperatures were tested: 30°C (♦), 32°C (■), 34°C (▲) and 37°C (●). Error bars represent the deviations from two independent measurements.