Figure 2

Evaluation of the Δpah1 growth phenotype. The growth phenotype of an empty vector strain and the Δpah1 strain was compared on glucose and oleic acid as carbon sources. The empty vector strain (EV) is the PO1d ΔOCH1 strain transformed with a plasmid containing the LEU selection marker cassette to rule out any effect of the selection pressure, compared to the PAH1 knockout strain (Δpah1), which carries the LEU selection marker on the knockout construct. A. Growth on glucose as carbon source for 27 hours. Growth of the knockout was retarded only slightly and in the stationary phase, cells reached the same OD₆₀₀ as the wild type strain. B. Growth on oleic acid as carbon source for 55 hours. Also here, the PAH1 knockout strain has a prolonged lag phase but it reaches the stationary phase at the same OD₆₀₀ as the empty vector strain.