High-level intracellular expression of heterologous proteins in Brevibacillus choshinensis SP3 under the control of a xylose inducible promoter

Table 1 Plasmids used in this study

Name	Expression	Promoter	Antibiotic resistance	Tag
GFP-pNI	Intracellular	P2	neomycin	N-term His-Tag
GFP-pNC	Extracellular	P2	neomycin	N-term His-Tag
GFP-pHis1522	Intracellular	PxylA	tetracycline	N-term His-Tag
SEC-GFP-pHis1522	Extracellular	PxylA	tetracycline	No
α-amylase-pNI	Intracellular	P2	neomycin	N-term His-Tag
α-amylase-pNC	Extracellular	P2	neomycin	No
α-amylase-pHis1522	Intracellular	PxylA	tetracycline	N-term His-Tag
SEC- α-amylase-pHis1522	Extracellular	PxylA	tetracycline	No
TcdA-GT-pNI	Intracellular	P2	neomycin	N-term His-Tag
TcdA-GT-pHis1522	Intracellular	PxylA	tetracycline	N-term His-Tag

ISSN: 1475-2859