Figure 3

Time course of extracellular GFP production by Brevibacillus choshinensis SP3 at different temperatures. A) GFP fluorescence of culture supernatant of Brevibacillus carrying GFP-pNC grown at 37°C (red line), 30°C (green line), 25°C (blue line) B) GFP fluorescence of culture supernatant of Brevibacillus carrying the SEC-GFP-pHis1522 grown at 37°C (red line), 30°C (green line), 25°C (blue line) C) Western blot of the culture supernatant of Brevibacillus carrying GFP-pNC grown at 25°C D) Western blot of the culture supernatant of Brevibacillus carrying SEC-GFP-pHis1522 grown at 25°C. SEC-GFP-pHis1522 was induced with 0,5% xylose. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations.