Figure 2

Time course of intracellular α-amylase and TcdA-GT production by Brevibacillus choshinensis SP3 at different temperatures. A) α-amylase activity of Brevibacillus lysate carrying α-amylase-pNI and α-amylase-pHis1522 grown in at 37°C (red line), 30°C (green line), 25°C (blue line) B) Western blot of Brevibacillus cells carrying α-amylase-pNI and α-amylase-pHis1522 grown at 25°C C) Western blot of Brevibacillus cells carrying TcdA-GT-pNI and TcdA-GT-pHis1522 grown at 25°C α-amylase-pHis1522 and TcdA-GT-pHis1522 were induced with 0,5% of xylose. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations.