Figure 1

Time course of intracellular GFP production by Brevibacillus choshinensis SP3 at different temperatures. A) GFP fluorescence of Brevibacillus cells carrying GFP-pNI grown at 37°C (red line), 30°C (green line), 25°C (blue line) B) GFP fluorescence of Brevibacillus cells carrying the GFP-pHis1522 grown at 37°C (red line), 30°C (green line), 25°C (blue line) C) Western blot analysis of Brevibacillus cells carrying GFP-pNI grown at 30°C D) Western blot of Brevibacillus cells carrying GFP-pHis1522 grown at 30°C E) Fluorescence imagine of Brevibacillus cells carrying GFP-pNI and GFP-pHis1522 after 48 h of growth at 37°C, 30°C, 25°C (about 20 OD₆₀₀). The GFP-pHis1522 was induced with 0,5% of xylose. 5 mL of culture were centrifuged and the image was carried out from the cell pellet using ImageQuant 400 (GE Healthcare). All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations.