Table 3 Primers used in this study
Target genes | Primers | Restriction enzymes |
|---|---|---|
ILV5c (fw) | 5′-ttttCCTAGGatgttgaagcaaatcaacttcggtggtact | Avr II |
ILV5c (rv) | 5′-ttttGGCCGGCCttattggttttctggtctcaactttctg | Fse I |
ILV3c (fw) | 5′-aaaaGTCGACatgctttatgccaccggtttcaagaaggaa | Sal I |
ILV3c (rv) | 5′-ggggGCGGCCGCtcaagcatctaaaacacaaccgttggaa | Not I |
ILV2c (fw) | 5′-ccccCCCGGGatgccagagcctgctccaagtttcaatgtt | Xma I |
ILV2c (rv) | 5′-ttttGGCGCGCCtcagtgcttaccgcctgtacgcttatga | Asc I |
MDH2 (fw) | 5′-aaaaCCTAGGatgcctcactcagttacacc | Avr II |
MDH2 (rv) | 5′-aaaaGGCCGGCCttaagatgatgcagatctcg | Fse I |
sMAE1 (fw) | 5′-ttttGTCGACatgtggcctattcagcaatcgcg | Sal I |
sMAE1 (rv) | 5′-ttttGCGGCCGCctacaattggttggtgtgca | Not I |
MAE1 (fw) | 5′-ccccGTCGACatgcttagaaccagactatc | Sal I |
MAE1 (rv) | 5′ttttGCGGCCGCctacaattggttggtgtgca | Not I |
PYC2 (fw) | 5′-aaaaCCCGGGatgagcagtagcaagaaatt | Xma I |
PYC2 (rv) | 5′-aaaaGGCGCGCCttactttttttgggatgggg | Asc I |
ADH6 (fw) | 5′ggggCCTAGGatgtcttatcctgagaaa | Avr II |
ADH6 (rv) | 5′-aaaaGGCCGGCCctagtctgaaaattctttgt | Fse I |
kivd (fw) | 5′-ccccGTCGACatgtatacagtaggagatta | Sal I |
kivd (rv) | 5′-ccccGCGGCCGCttatgatttattttgttcag | Not I |