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Table 1 Yeast strains constructed in this study

From: Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance

Strains

Genotypes

YPH499

MAT a ura3-52 lys2-801 ade2-101 trp1- Δ63 his3- Δ200 leu2- Δ1

BY4741

MAT a his3 Δ1 leu2 Δ0 met15 Δ0 ura3 Δ0

BSW100

BY4741 /pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pda1Δ

BY4741 pda1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pdb1Δ

BY4741 pdb1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 lat1Δ

BY4741 lat1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 lpd1Δ

BY4741 lpd1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 irc15Δ

BY4741 irc15Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pdc5Δ

BY4741 pdc5Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pdc6Δ

BY4741 pdc6Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 thi3Δ

BY4741 thi3Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 dld1Δ

BY4741 dld1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pyc1Δ

BY4741 pyc1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 pyc2Δ

BY4741 pyc2Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW100 mae1Δ

BY4741 mae1Δ/pGK423-kivd/pGK425-ILV2/pGK426-ADH6

BSW101

BY4741/pGK423/pGK425/pGK426

BSW101 pda1Δ

BY4741 pda1Δ/pGK423/pGK425/pGK426

BSW101 pdb1Δ

BY4741 pdb1Δ/pGK423/pGK425/pGK426

BSW101 lat1Δ

BY4741 lat1Δ/pGK423/pGK425/pGK426

BSW101 lpd1Δ

BY4741 lpd1Δ/pGK423/pGK425/pGK426

BSW4

YPH499/pATP426-kivd-ADH6/pATP423

BSW5

YPH499/pATP426-kivd-ADH6/pATP423-sMAE1

BSW6

YPH499/pATP426-kivd-ADH6/pATP423-MsM

BSW7

YPH499/pATP426-kivd-ADH6/pATP423-PMsM

BSW8

YPH499/pATP426-kivd-ADH6/pATP423-MAE1

BSW9

YPH499/pATP426-kivd-ADH6/pATP423-MM

BSW10

YPH499/pATP426-kivd-ADH6/pATP423-PMM

BSW13

YPH499/pATP426-kivd-ADH6/pATP425/pATP423

BSW14

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423

BSW15

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-sMAE1

BSW16

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-MsM

BSW17

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-PMsM

BSW18

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-MAE1

BSW19

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-MM

BSW20

YPH499/pATP426-kivd-ADH6/pILV2L/pATP423-PMM

BSW187

BY4741/pATP426-kivd-ADH6-ILV2/pILV532cytM

BSW191

BY4741/pATP426-kivd-ADH6-ILV2/pILV532cytM/pATP423-PMsM

BSW192

BY4741/pATP426-kivd-ADH6-ILV2/pILV532cytM/pATP423-MAE1

BSW205

BY4741 lpd1Δ/pATP426-kivd-ADH6-ILV2/pILV532cytM/pATP423-MAE1

BSW206

BY4741 lpd1Δ/pATP426-kivd-ADH6-ILV2/pILV532cytM/pATP423-PMsM

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Microbial Cell Factories

ISSN: 1475-2859

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