Table 2 Plasmids and strains
From: Photoautotrophic production of D-lactic acid in an engineered cyanobacterium
Plasmids/Strains | Description | Source or reference |
|---|---|---|
Plasmids | ||
pUC57-glda_sth | Chemically synthesized gene cassette consisting of Ptrc, gldA101-syn and sth. | |
pQZ115 | Plasmid carrying gldA101 | [20] |
pTKA3 | Backbone plasmid for all vectors constructed in this study, with psbA1 as the integration loci. | [32] |
pYY1 | Derived from pTKA3 with gldA101 and the promoter, Ptrc. | This study |
pDY2 | Derived from pTKA3 with gldA101-syn, sth and the promoter, Ptrc. | This study |
pDY3 | Derived from pTKA3 with gldA101-syn and the promoter, Ptrc. | This study |
Strains | ||
E. coli 10-Beta | Cloning host strain. | New England Biolabs |
Synechocystis sp. PCC 6803 | Glucose tolerant wild type, naturally competent. | This study |
AV08 | Synechocystis Ptrc::gldA101::Kmr, GlyDH* of Bacillus. | This study |
AV10 | Synechocystis Ptrc::(gldA101-syn)-sth::Kmr, GlyDH* of Bacillus, transhydrogenase of Pseudomonas. | This study |
AV11 | Synechocystis Ptrc::gldA101-syn::Kmr, GlyDH* of Bacillus. | This study |