Table 1 Primer sequences
From: Photoautotrophic production of D-lactic acid in an engineered cyanobacterium
Primer name | Sequence (5′ → 3′) |
|---|---|
gldA-o-F | GGATCCTTGACAATTAATCATCCGGCTCG |
gldA-o-F2 | GGATCCTTGACAATTAATCATCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAGATATAATCATATGACGAAAATCATTACCTCTCCAAGCAAGTTTATACAAGG |
gldA-o-F3 | ATGACGAAAATCATTACCTCTCCAAG |
gldA-o-R | GCTAGCTCATGCCCATTTTTCCTTATAATACCGCCCG |
gldA-o-R2 | TTAGGCCCACTTTTCCTTGTAATAGC |
tranNADH-F | CCTAAGCTAGCGGAGGACTAGCATGG |
tranNADH-R | GCTAGCGGTACCTCAAAAAAGCCGG |
ptka3-F | CCCGAAGTGGCGAGCCCGAT |
CO-F | TTGATGTTGCCTTTGAACCC |
O-F | ATGGATACGAAAGTGATTGC |
sth-F | GAGCTACCACCTGCGCAACA |
AMV17R | GCGCGACTCCCCGTCTTTGACTATCCTTTTTAGGATGGGGCA |
ps1_up_fwda | TACCGGAACAGGACCAAGCCTT |