Skip to main content

Table 1 Chemico-physical parameters of the recombinant proteins as produced upon cloning in pET32b or pColdI

From: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

Protein Length (aa) Molecular mass (Da) Theoretical pI
Trx-TB10.4 261 28,133.6 5.06
TB10.4 103 11,076.3 4.44
Trx-Ag85B 450 48,405.2 5.11
Ag85B 292 31,347.9 4.77
Trx-full 546 58,777.7 4.99
His-full 1 414 44,865.9 5.11
full 1 388 41,720.5 4.71
His-full 2 397 43,060.0 5.53
  1. Theoretical isoelectric point (pI) was calculated by ExPASyBioinformatic Resource Portal (http://www.expasy.org/) using ProtParam tool.