Table 1 Chemico-physical parameters of the recombinant proteins as produced upon cloning in pET32b or pColdI
Protein | Length (aa) | Molecular mass (Da) | Theoretical pI |
|---|---|---|---|
Trx-TB10.4 | 261 | 28,133.6 | 5.06 |
TB10.4 | 103 | 11,076.3 | 4.44 |
Trx-Ag85B | 450 | 48,405.2 | 5.11 |
Ag85B | 292 | 31,347.9 | 4.77 |
Trx-full | 546 | 58,777.7 | 4.99 |
His-full 1 | 414 | 44,865.9 | 5.11 |
full 1 | 388 | 41,720.5 | 4.71 |
His-full 2 | 397 | 43,060.0 | 5.53 |