Figure 8

Analysis of the T-cell reactivity against Ag85B, TB10.4 and His- full 2 proteins and positive control superantigen (SEB). Control (white boxes) and active-TB (grey boxes) PBMCs were tested in ELISPOT and the IFN-γ positive effector T-cells enumerated and referred to 1×10⁶ PBMCs (ordinate). The number of spots observed in absence of antigen was subtracted. Data are reported as median, 25–75 percentile range (box), and minimum and maximum (line) observed in subject groups of the study population presenting an immunological valid test (active-TB n = 10; healthy controls n = 6).