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Figure 5 | Microbial Cell Factories

Figure 5

From: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

Figure 5

Time course of pH (, solid line), dO 2 (♦, solid line) and OD 600nm (, dashed line) in 3 L batch cultivation trials of recombinant E. coli BL21(DE3) cells producing M. tuberculosis immunogenic proteins. A) E. coli BL21(DE3) cells containing pET32b-Trx-TB10.4 plasmid and grown in LB medium. B) E. coli BL21(DE3) cells containing pET32b-Trx-Ag85B plasmid and grown in SB medium. C) E. coli BL21(DE3) cells containing pColdI-His-full 2 and growing in SB/NaCl medium. Temperature (■, dashed line) was kept constant at 37°C before IPTG addition and then reduced to 18°C for Trx-TB10.4 and Trx-Ag85B production, and at 15°C for His-full 2 production, until harvest. The induction of protein expression was done at OD600nm = 0.8 with 0.1 mM IPTG for Trx-TB10.4, at OD600nm = 2 with 0.1 mM IPTG for Trx-Ag85B, and at OD600nm = 2 with 0.1 mM IPTG for His-full 2. Biomass (wet weight) collected at the harvest time was 8.5 g/L for Trx-TB10.4, 19.5 g/L for Trx-Ag85B and 6.5 g/L for His-full 2.

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