Bacterial cell factories for recombinant protein production; expanding the catalogue

Ferrer-Miralles, Neus; Villaverde, Antonio

doi:10.1186/1475-2859-12-113

Microbial Cell Factories

Table 1 The most important bacterial groups explored as cell factories for recombinant protein production

From: Bacterial cell factories for recombinant protein production; expanding the catalogue

	Host	Main features	Reviews^a	Main bacterial Species	Case proteins	References
Proteobacteria	Caulobacteria	Easy purification of secreted RSaA fusions	[31, 32]	Caulobacter crescentus	Hematopoietic necrosis virus capsid proteins	[33]
					β-1,4-glycanase	[34]
	Phototrophic bacteria	High production of membrane proteins	[35]	Rodhobacter sphaeroides	Membrane proteins	[35]
	Cold adapted bacteria	Improved protein folding	[36, 37]	Pseudoalteromonas haloplanktis	3H6 Fab	[38]
					Human nerve growth factor	[39]
				Shewanella sp. strain Ac10	β-Lactamase, peptidases, glucosidase	[40]
	Pseudomonads	Efficient secretion	[41]	Pseudomonas fluorescens	Human granulocyte colony-stimulating factor	[42]
				Pseudomonas putida	Single chain Fv fragments	[43]
				Pseudomonas aeruginosa	Penicillin G acylase	[44]
	Halophilic bacteria	Solubility favored	[45]	Halomonas elongata	β-Lactamase	[45]
				Chromohalobacter salexigens	Nucleoside diphosphate kinase	[46]
Actinobacteria	Streptomycetes	Efficient secretion	[47, 48]	Streptomyces lividans	M. tuberculosis antigens	[49]
				Streptomyces griseus	Trypsin	[50]
	Nocardia	Efficient secretion	[48]	Nocardia lactamdurans	Lysine-6-aminotransferase	[51]
	Mycobacteria	Posttranslational modifications	[52]	Mycobacterium smegmatis	Hsp65-hIL-2 fusion protein	[53]
					Mycobacterial proteins	[54]
	Coryneform bacteria	High-level production and secretion; GRAS	[48, 55]	Corynebacterium glutamicum	Protein-glutaminase	[56]
				Corynebacterium ammoniagenes	Pro-transglutaminase	[57]
				Brevibacterium lactofermentum	Cellulases	[58]
Firmicutes	Bacilli	High-level production and secretion	[59–64]	Bacillus subtilis	β-Galactosidase	[65]
				Bacillus brevis	Disulfide isomerase	[66, 67]
				Bacillus megaterium	Antibodies	[68]
				Bacillus licheniformis	Subtilisin	[69]
				Bacillus amyloliquefaciens	Amylases	[70]
	Lactic acid bacteria	Secretion; GRAS	[22–24, 71]	Lactococcus lactis	Fibronectin-binding protein A, internalin A, GroEL	[72, 73]
				Lactobacillus plantarum	β-Galactosidase	[74]
				Lactobacillus casei	VP2-VP3 fusion protein of infectious pancreatic necrosis virus	[75]
				Lactobacillus reuteri	Pediocin PA-1	[76]
				Lactobacillus gasseri	CC chemokines	[77]

^aGeneric reviews about the biological platform or about specific tools for protein production.

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ISSN: 1475-2859

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