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Table 4 Oligonucleotides used in this study

From: Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a

Name

Sequence

NS3-F

5'-TATAGGATCC ATGCACCATCACCATCATCACGCCCCGATCACAGCATAC-3'

Bam HI

NS3-R

5'-GAGCAAGCTT TTAGGTGGTTACTTCCAGATCAG-3'

Hind III

NS5A-F

5'-GGAATTCCATATG AGCGGTGATTGGCTGCGTA-3'

Nde I

NS5A-R

5'-TCAAAGCTT TTAGTGGTGATGGTGATGATGGCAGCAGACCACGCTCTGCT-3'

HindIII

NS5A-T-F

5'-GGAATTCCATATG CACCATCACCACCATCACCCTGGGCTGCCCTTTATTTCCT-3'

Nde I

NS5A-T-R

5'-TCAAAGCTT TTAGCAGCAGACCACGCTCTGCT-3'

Hind III

NS3-cDNA

5'-ACCCCTCCAAGCAACACCCA-3'

NS5A-cDNA

5'-ATGTTGCTGCCCATCTCTTG-3'

  1. Nucleotides shown as italicized represent the respective restriction sites used for cloning purposes whereas underlined nucleotides sequence encode for oligohistidine tag, incorporated into genes of interest for downstream purification processes.