Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a

Table 3 Secondary structure analysis of His ₆ -NS3 and NS5A-T-His ₆ by FT-IR, CD spectroscopy and homology modeling

Protein	Method	α-helix (%)	β-sheet (%)
NS3	FT-IR¹	25.7	34.6
	CD²	24.4	46
	CD³	11	37.6
	Homology model⁴	25	30
NS5A-T	FT-IR¹	29	28
	CD²	9.1	32.7
	CD³	18	37

¹Bands contributing (FT-IR spectra) to each type of secondary structure are indentified in Figure 7B and 7D respectively. Bands assignments for interpretation of spectra were made on the basis of previous measurements [48, 58]. ²CD spectra shown in Figure 5 were analyzed using protein concentration independent method at web based server http://perry.freeshell.org/raussens.html [52]. ³CD spectra were analyzed using DichroWeb server at http://dichroweb.cryst.bbk.ac.uk/html/home.shtml [53, 54] and program CONTIN/reference set 3 [55]. ⁴Secondary structure are estimated from the homology model of NS3/3a, built using the x-ray structure of NS3/1a (PDB ID: 3o8b) as a template [49].

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