Table 3 Description of the C glutamicum strains and plasmids used in the present work for heterologous production of ectoine and hydroxyectoine
Strain | Description | Reference |
|---|---|---|
C. glutamicum LYS-1 | C. glutamicum ATCC 13032 + amino acid exchange T311I in the lysC gene (NCgl0247), encoding aspartokinase | [53] |
C. glutamicum ECT-1 | LYS-1 + genome-based integration of the codon-optimized biosynthetic ectoine cluster of P. stutzeri into the ddh gene (NCgl2528), encoding diaminopimelate dehydrogenase [ddh: tuf p -ectABCD] | This work |
C. glutamicum ECT-2 | ECT-1 + deletion of lysE gene (NCgl1214), encoding the lysine exporter | This work |
Plasmids | Description | Reference |
pTC | Expression vector for DNA-methyltransferase of C. glutamicum, ORI for E. coli and tetracycline resistance as selection marker. Used in E. coli NM522 to add the C. glutamicum-specific DNA-methylation pattern to the integrative transformation vector. | [53] |
pClik int sacB | Integrative transformation vector for C. glutamicum with MCS, ORI for E. coli, and KanR and sacB as selection markers. | [57] |
pClik int sacB ectABCD | Integrative transformation vector for the integration of the codon-optimized biosynthetic ectoine/hydroxyectoine gene cluster derived from P. stutzeri A1501 into the ddh locus of C. glutamicum | This work |
pClik int sacB ΔlysE | Integrative transformation vector for deletion of the lysine exporter lysE | [65] |