Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine

Table 2 Concentration of free intracellular amino acids of the aspartate-family and of intracellular ectoine of the lysine-producing C. glutamicum strains LYS-1 and its ectoine-producing derivatives C. glutamicum ECT-1 and C. glutamicum ECT-2

Strain	Aspartate [μmol g_cdm^-1]	Asparagine [μmol g_cdm^-1]	Threonine [μmol g_cdm^-1]	Lysine [μmol g_cdm^-1]	Ectoine [μmol g_cdm^-1]
(A)
C. glutamicum LYS-1	11.8 ± 3.3	0.7 ± 0.3	5.1 ± 0.9	23.4 ± 3.1	--
C. glutamicum ECT-1	8.1 ± 2.9	24.6 ± 6.0	3.9 ± 0.6	14.5 ± 4.1	126.8 ± 25.5
C. glutamicum ECT-2	7.0 ± 0.3	20.1 ± 4.9	3.0 ± 0.9	76.7 ± 11.3	34.1 ± 14.2
(B)
C. glutamicum LYS-1	9.1 ± 0.9	1.4 ± 0.2	6.8 ± 0.5	24.4 ± 6.3	--
C. glutamicum ECT-1	7.6 ± 0.6	28.1 ± 4.0	6.5 ± 0.4	16.3 ± 2.0	158.5 ± 20.7
C. glutamicum ECT-2	7.9 ± 0.6	17.0 ± 2.4	5.2 ± 0.4	52.8 ± 12.6	36.1 ± 7.6

Cells were grown at 30°C (A) and 35°C (B), respectively in mineral salt medium. The data represent mean values with standard deviations from two biological replicates, each sampled at three different optical densities (OD 2, OD 4 and OD 8).

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