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Table 1 Growth and production performance of the C. glutamicum strains LYS-1 [53], ECT-1 and ECT-2 during batch cultivation on a mineral salt medium with glucose as carbon source at 30°C (LYS-1, ECT-1, ECT-2) and 35°C (ECT-2)

From: Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine

 

C. glutamicum LYS-1

C. glutamicum ECT-1

C. glutamicum ECT-2

C. glutamicum ECT-2

Temperature

30°C

30°C

30°C

35°C

Rates

μ [h-1]

0.38 ± 0.01

0.34 ± 0.00

0.36 ± 0.00

0.34 ± 0.02

qs [mmol g-1 h-1]

4.86 ± 0.10

3.82 ± 0.08

3.51 ± 0.05

3.74 ± 0.10

qEct [mmol g-1 h-1]

-

0.07 ± 0.01

0.09 ± 0.01

0.12 ± 0.01

qLys [mmol g-1 h-1]

0.39 ± 0.02

0.20 ± 0.01

0.00 ± 0.00

0.00 ± 0.00

Yields

YX/S [g mol-1]

82.1 ± 1.3

87.2 ± 2.8

101.5 ± 0.6

90.9 ± 6.3

YEct/S [mmol mol-1]

-

19.4 ± 1.5

24.6 ± 0.6

32.0 ± 0.8

YLys/S [mmol mol-1]

81.2 ± 3.2

53.8 ± 2.5

0.0 ± 0.0

0.0 ± 0.0

YTre/S [mmol mol-1]

9.4 ± 0.4

5.6 ± 0.1

7.2 ± 0.9

6.7 ± 0.2

YAKG/S [mmol mol-1]

0.0 ± 0.0

2.6 ± 0.4

1.0 ± 0.1

1.9 ± 0.1

YEctOH/S [mmol mol-1]

-

0.6 ± 0.0

0.6 ± 0.0

0.9 ± 0.1

  1. The data represent mean values and standard deviations from three biological replicates and denote the specific rates for growth (μ), substrate uptake (qS), and product formation (qP). Additionally, the yield for biomass (YX/S), ectoine (YEct/S), lysine (YLys/S), trehalose (YTre/S), α-ketoglutarate (YAKG/S), and hydroxyectoine (YEctOH/S) are given.